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TransIT-X2® Dynamic Delivery System Technical Support│Toll-free (U.S.) 844.MIRUSBIO│Direct: 608.441.2852││ Protocol for MIR 6000, 6003, 6004, 6005, 6006 Quick Reference Protocol, MSDS and Certificate of Analysis available at /6000 INTRODUCTION TransIT-X2® Dynamic Delivery System is an advanced, non-liposomal polymeric system that enables high efficiency transfection of many cell types, including primary cells. TransIT-X2 can be used for DNA delivery (Page 2), siRNA delivery (Page 4) or simultaneous delivery of DNA and siRNA (Page 7). Transfections with TransIT-X2 do not require medium changes and can be carried out in serum-containing medium. SPECIFICATIONS MATERIALS Materials Supplied TransIT-X2 Dynamic Delivery System is supplied in the following formats. Product No. Quantity MIR 6003 1 × 0.3 ml MIR 6004 1 × 0.75 ml MIR 6000 1 × 1.5 ml MIR 6005 5 × 1.5 ml MIR 6006 10 × 1.5 ml Materials Required, but not Supplied  Cultured cells  Appropriate cell culture medium  Nucleic acid (plasmid DNA and/or siRNA)  Serum-free medium (e.g. Opti-MEM® I Reduced-Serum Medium)  Sterile tube for transfection complex preparation  Micropipets  Reporter assay as required  Optional: Selection antibiotic (e.g. G418 or Hygromycin B) for stable transfection