NUCLEODUR C18 Gravity 液相色谱柱
C18 Gravity:PH稳定应用范围宽达1.0—11.0
1、 满足您对方法开发 2、3、端PH应用的需求 和复杂样品的分离
Gravity是在NUCLEODUR超纯硅胶基础上,采用 18%高碳载量和多步交联键合技术。特别适合强碱性样品的分析,而且能在PH=1.0—11.0范围内稳定使用,符合美国药典USP L1,常卖货号760101.46 C18 Gravity 4.6X250mm 5um
主要特点:
适用于液相色谱/质谱和液相色谱法在PH(PH值1 - 11)
优越的基失活
方法开发的理想
技术特点:
十八烷基改性高密度、多封端
孔径110A;粒径1.8μm,3 m和5 mμ
10,7,12和16μm粒子的制备要
碳含量18%
推荐应用:
整体复杂的分析分离
复合类分离包括药物,如镇痛药、抗炎药、抗抑郁药;除草剂;植物药物;免疫抑制剂
Standard program* of columns packed with NUCLEODUR? C18 Gravity |
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Column system |
粒径: |
1.8 μm |
3 μm |
5 μm |
10 μm |
|
分析柱 |
SHAPE \* MERGEFORMAT |
SHAPE \* MERGEFORMAT |
+ |
SHAPE \* MERGEFORMAT |
+ |
|
制备 |
SHAPE \* MERGEFORMAT |
SHAPE \* MERGEFORMAT |
+ |
液相色谱分析法
订货信息:
Length -> |
30 mm |
50 mm |
75 mm |
100 mm |
125 mm |
150 mm |
250 mm |
NUCLEODUR? C18 Gravity, 1.8 μm |
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2 mm ID |
760078.20 |
760079.20 |
760071.20 |
760076.20 |
760075.20 |
||
3 mm ID |
760078.30 |
760079.30 |
760076.30 |
||||
4 mm ID |
760078.40 |
760079.40 |
760076.40 |
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4.6 mm ID |
760078.46 |
760079.46 |
760076.46 |
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EC guard columns* |
4 x 2 mm |
761901.20 |
4 x 3 mm |
761901.30 |
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NUCLEODUR? C18 Gravity, 3 μm |
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2 mm ID |
760080.20 |
760084.20 |
760081.20 |
760083.20 |
760082.20 |
||
3 mm ID |
760080.30 |
760084.30 |
760081.30 |
760083.30 |
760082.30 |
||
4 mm ID |
760080.40 |
760084.40 |
760081.40 |
760083.40 |
760082.40 |
||
4.6 mm ID |
760080.46 |
760086.46 |
760084.46 |
760081.46 |
760083.46 |
760082.46 |
|
EC guard columns* |
4 x 2 mm |
761902.20 |
4 x 3 mm |
761902.30 |
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NUCLEODUR? C18 Gravity, 5 μm |
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2 mm ID |
760102.20 |
760104.20 |
760100.20 |
760103.20 |
760101.20 |
||
3 mm ID |
760102.30 |
760104.30 |
760100.30 |
760103.30 |
760101.30 |
||
4 mm ID |
760102.40 |
760104.40 |
760100.40 |
760103.40 |
760101.40 |
||
4.6 mm ID |
760102.46 |
760106.46 |
760104.46 |
760100.46 |
760103.46 |
760101.46 |
|
EC guard columns* |
4 x 2 mm |
761903.20 |
4 x 3 mm |
761903.30 |
Guard columns for EC columns |
EC columns with ID |
Use guard |
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2 mm |
3 mm |
4 mm |
4.6 mm |
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* |
Column Protection System |
EC guard column |
4/2 |
4/3 |
4/3 |
4/3 |
REF 718966 |
VarioPrep 柱用在液相制备
Length |
10 mm ID |
21 mm ID |
32 mm ID |
40 mm ID |
NUCLEODUR? C18 Gravity, 5 μm |
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50 mm |
762103.100 |
762103.210 |
||
125 mm |
762109.100 |
762109.210 |
||
150 mm |
762100.400 |
|||
250 mm |
762113.100 |
762113.210 |
762113.320 |
762113.400 |
NUCLEODUR? C18 Gravity, 10 μm |
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250 mm |
762250.210 |
762250.400 |
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VP guard columns with NUCLEODUR? C18 Gravity |
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8 mm ID |
16 mm ID |
32 mm ID |
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10 mm* |
762160.80 |
762160.160 |
||
15 mm* |
762163.320 |
* VP guard column |
Size |
Pack of |
for VP column |
required holder |
REF |
VP 10/8 |
10 x 8 mm ID |
2 |
10 mm ID |
8 mm |
718251 |
VP 10/16 |
10 x 16 mm ID |
2 |
21 mm ID |
16 mm |
718256 |
VP 15/32 |
15 x 32 mm ID |
1 |
32–40 mm ID |
32 mm |
718253 |
对照品舒巴坦钠的标定
使用NUCLEODUR C18 Gravity和对照柱X按照《药典》方法检测进行标定
色谱柱:NUCLEODUR C18 Gravity 250×4.6mm 5um 对照柱X 250×4.6mm 5um
流动相:0.005 mol/L 四丁基氢氧化铵(PH=5.0):乙腈=75:25
温 度:35℃
检测器:UV 230nm
结果:
|
舒巴坦钠理论塔板数 |
舒巴坦钠 |
重现性 |
C18 Gravity |
7398 |
4.13 |
0.71 |
对照柱X |
6317 |
4.00 |
0.15 |
应用:
1、 万古霉素
Substances: |
vancomycin |
|
Column: |
EC 250/4.6 NUCLEODUR® C18 Gravity, 5 µm |
|
Phase: |
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REF number: |
760101.46 |
|
Sample pretreatment: |
Sample concentration: 2
mg/mL in eluent A |
|
Conditions: |
Eluent A: 920 mL stock
solution + 10 mL THF + 70 mL ACN |
|
Detection: |
UV, 280 nm |
|
Author(-s): |
HPLC department |
|
Source: |
MACHEREY-NAGEL, Germany 2010 |
|
Keywords: |
antibiotics; pharmacopeia; Pharm. Eu.; asymmetry factor |
|
Legend: |
1 - vancomycin |
|
Note: |
Vancomycin is determined with an asymmetry factor of 1.12 (Pharm. Eu < 1.60) |
Substances: |
acetylsalicylic acid; sulindac; piroxicam; suprofen; tolmetin; naproxen; diflunisal; fenoprofen; flurbiprofen; niflumic acid; indomethacin; ibuprofen |
|
Column: |
EC 250/4.6 NUCLEODUR® C18 Gravity, 5 µm |
|
Phase: |
NUCLEODUR® C18 Gravity |
|
MN catalog number: |
760101.46 |
|
Conditions: |
Mobile
phase: aceto |
|
Detection: |
UV, 230 nm |
|
Author(-s): |
HPLC department |
|
Source: |
MACHEREY-NAGEL, Germany 2008 |
|
Keywords: |
antiinflamatoray drugs; drugs; NSAID |
|
Legend: |
1
- acetylsalicylic acid |
Substances: |
coumafuryl; warfarin; pindone; coumatetralyl; coumachlor; diphacinone; chlorphacinone; bromadiolone i; difenacoum; bromadiolone ii; flocoumafen i; flocumafen ii; brodifacoum; difethialone; pyranocoumarin |
|
Column: |
EC
250/4.6 NUCLEODUR® C18 Gravity, 5 μm |
|
Phase: |
NUCLEODUR® C18 Gravity |
|
REF number: |
760101.46 |
|
Conditions: |
Buffer: |
|
Detection: |
A)
DAD UV, 308 nm (285 nm, 260 nm) |
|
Author(-s): |
Freidhof, K. |
|
Source: |
Landesuntersuchungsamt Rheinland-Pfalz, Koblenz; Germany |
|
Keywords: |
anti-coagulant drugs; drugs |
|
Legend: |
Peaks: |